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Aliquots were injected into the HPLC column. Amounts of 7-hydroxyamoxapine and residual amoxapine were determined by comparing the peak areas of these compounds with the peak areas of known concentrations of amoxapine. For NMR analysis Table 1, Fig. 2 ; , each metabolite was dissolved in 0.5 ml of methylene-d2-chloride 99.96 atom% 2 H ; . The NMR measurements were obtained at 500.13 MHz with a model AM500 spectrometer Bruker Instruments, Billerica, Mass. ; . Chemical shifts were determined on the scale parts per million ; by assigning the residual solvent peak to 5.32 ppm. Typical data acquisition parameters were as follows: data size, 32 K; sweep width, 7, 042 Hz; filter width, 8, 900 Hz; acquisition time, 2.33 s; flip angle, 90; relaxation delay, 0 s; and temperature, 301 K. Mass spectral analyses were performed as previously described 23 ; . However, electron ionization EI ; analyses Table 1 ; were performed with an electron energy of 25 V enhance the molecular ion region of the mass spectra. Molecular.
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Blood has two major components: Plasma is a clear yellow liquid that contains proteins, nutrients, hormones, electrolytes, and other substances. It constitutes about 55% of blood. White and red blood cells and platelets make up the balance of blood. The white cells are the infection fighters for the body, and platelets are necessary for blood clotting. The important factors in anemia, however, are red blood cells. Red blood cells RBCs ; , also known as erythrocytes, carry oxygen throughout the body to nourish tissues and sustain life. Red blood cells are the most abundant cells in our bodies; men have about 5, 200, 000 and women have about 4, 700, 000 per cubic millimeter of blood. To understand red blood cells and their role in anemia, it is useful to know certain facts about them. Hemoglobin and Iron Each red blood cell contains between 200 and 300 hemoglobin molecules. Hemoglobin is a complex molecule and the most important component of red blood cells. It is composed of protein globulin ; and a molecule heme ; , which binds to iron. In the lungs, the heme component binds to oxygen in exchange for carbon dioxide. The oxygenated red blood cells are then transported to the body's tissues, where the hemoglobin releases the oxygen in exchange for carbon dioxide, and the cycle repeats. The oxygen is used in the mitochondria, the power source within all cells. Red blood cells typically circulate for about 120 days before they are broken down in the spleen. Most of the iron present in hemoglobin can be recycled and reused. Structure and Shape Red blood cells are extremely small and look something like tiny, flexible inner tubes. This unique shape offers many advantages: It provides a large surface area to absorb oxygen and carbon dioxide. Its flexibility allows it to squeeze through capillaries, the tiny blood vessels that join the arteries and veins. Abnormally shaped or sized erythrocytes are typically destroyed and eliminated. Blood Cell Production Erythropoiesis ; The actual process of making red blood cells is called erythropoiesis. In Greek, erythro means "red" and poiesis means "the making of things." ; The process of manufacturing, recycling, and regulating the number of red blood cells is complex and involves many parts of the body: The body carefully regulates its production of red blood cells so that enough are manufactured to carry oxygen but not so many that the blood becomes thick or sticky viscous ; . Most of the work of erythropoiesis occurs in the bone marrow. In children younger than five years old, the marrow in all the bones of the body is enlisted for producing red blood cells. As a person ages, red blood cells are eventually produced only in the marrow of the spine, ribs, and pelvis. If the body requires an increase in oxygen at high altitudes, for instance ; , the kidney triggers the release of erythropoietin EPO ; , a hormone that acts in the bone marrow to increase the production of red blood cells. The life span of a red blood cell is between 90 and 120 days. Old red blood cells are removed from the blood by the liver and spleen. There they are broken down and iron is returned to the bone marrow to make new cells. Oxygen Loss in Red Blood Cells with Normal Hemoglobin In everyone, hemoglobin loses its oxygen normally in a number of ways: To sustain life, oxygen regularly passes from red blood cells to the tissues where it is needed to perform vital functions.
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